Publications

Rengarajan A, Goldblatt HE, Beebe DJ, Virumbrales-Muñoz M, Boeldt DS. Immune cells and inflammatory mediators cause endothelial dysfunction in a vascular microphysiological system. Lab Chip. 2024 Mar 12;24(6):1808-1820. doi: 10.1039/d3lc00824j. PubMed PMID: 38363157; PubMed Central PMCID: PMC11022267.

Abizanda-Campo S, Virumbrales-Muñoz M, Humayun M, Marmol I, Beebe DJ, Ochoa I, Oliván S, Ayuso JM. Microphysiological systems for solid tumor immunotherapy: opportunities and challenges. Microsyst Nanoeng. 2023;9:154. doi: 10.1038/s41378-023-00616-x. eCollection 2023. Review. PubMed PMID: 38106674; PubMed Central PMCID: PMC10724276.

Ayuso JM, Farooqui M, Virumbrales-Muñoz M, Denecke K, Rehman S, Schmitz R, Guerrero JF, Sanchez-de-Diego C, Campo SA, Maly EM, Forsberg MH, Kerr SC, Striker R, Sherer NM, Harari PM, Capitini CM, Skala MC, Beebe DJ. Author Correction: Microphysiological model reveals the promise of memory-like natural killer cell immunotherapy for HIV(±) cancer. Nat Commun. 2023 Nov 10;14(1):7292. doi: 10.1038/s41467-023-43057-w. PubMed PMID: 37949872; PubMed Central PMCID: PMC10638296.

Ayuso JM, Farooqui M, Virumbrales-Muñoz M, Denecke K, Rehman S, Schmitz R, Guerrero JF, Sanchez-de-Diego C, Campo SA, Maly EM, Forsberg MH, Kerr SC, Striker R, Sherer NM, Harari PM, Capitini CM, Skala MC, Beebe DJ. Microphysiological model reveals the promise of memory-like natural killer cell immunotherapy for HIV(±) cancer. Nat Commun. 2023 Oct 21;14(1):6681. doi: 10.1038/s41467-023-41625-8. PubMed PMID: 37865647; PubMed Central PMCID: PMC10590421.

Sanchez-de-Diego C, Virumbrales-Muñoz M, Hermes B, Juang TD, Juang DS, Riendeau J, Guzman EC, Reed-McBain CA, Abizanda-Campo S, Patel J, Hess NJ, Skala MC, Beebe DJ, Ayuso JM. Griddient: a microfluidic array to generate reconfigurable gradients on-demand for spatial biology applications. Commun Biol. 2023 Sep 9;6(1):925. doi: 10.1038/s42003-023-05282-3. PubMed PMID: 37689746; PubMed Central PMCID: PMC10492845.

Simitian G, Virumbrales-Muñoz M, Sánchez-de-Diego C, Beebe DJ, Kosoff D. Microfluidics in vascular biology research: a critical review for engineers, biologists, and clinicians. Lab Chip. 2022 Sep 27;22(19):3618-3636. doi: 10.1039/d2lc00352j. Review. PubMed PMID: 36047330; PubMed Central PMCID: PMC9530010.

Denecke KM, McBain CA, Hermes BG, Teertam SK, Farooqui M, Virumbrales-Muñoz M, Panackal J, Beebe DJ, Famakin B, Ayuso JM. Microfluidic Model to Evaluate Astrocyte Activation in Penumbral Region following Ischemic Stroke. Cells. 2022 Jul 31;11(15). doi: 10.3390/cells11152356. PubMed PMID: 35954200; PubMed Central PMCID: PMC9367413.

Ayuso JM, Virumbrales-Muñoz M, Lang JM, Beebe DJ. A role for microfluidic systems in precision medicine. Nat Commun. 2022 Jun 2;13(1):3086. doi: 10.1038/s41467-022-30384-7. Review. PubMed PMID: 35654785; PubMed Central PMCID: PMC9163169.

Burkel BM, Inman DR, Virumbrales-Muñoz M, Hoffmann EJ, Ponik SM. A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment. J Vis Exp. 2022 May 24;(183). doi: 10.3791/63413. PubMed PMID: 35695521; PubMed Central PMCID: PMC9327791.

Shapiro DD, Virumbrales-Muñoz M, Beebe DJ, Abel EJ. Models of Renal Cell Carcinoma Used to Investigate Molecular Mechanisms and Develop New Therapeutics. Front Oncol. 2022;12:871252. doi: 10.3389/fonc.2022.871252. eCollection 2022. Review. PubMed PMID: 35463327; PubMed Central PMCID: PMC9022005.

Virumbrales-Muñoz M, Ayuso JM, Loken JR, Denecke KM, Rehman S, Skala MC, Abel EJ, Beebe DJ. Microphysiological model of renal cell carcinoma to inform anti-angiogenic therapy. Biomaterials. 2022 Apr;283:121454. doi: 10.1016/j.biomaterials.2022.121454. Epub 2022 Mar 11. PubMed PMID: 35299086; PubMed Central PMCID: PMC9254636.

Virumbrales-Muñoz M, Ayuso JM. From microfluidics to microphysiological systems: Past, present, and future. Organs-on-a-chip. 2022 January. doi: https://doi.org/10.1016/j.ooc.2022.100015.

Lugo-Cintrón KM, Ayuso JM, Humayun M, Gong MM, Kerr SC, Ponik SM, Harari PM, Virumbrales-Muñoz M, Beebe DJ. Primary head and neck tumour-derived fibroblasts promote lymphangiogenesis in a lymphatic organotypic co-culture model. EBioMedicine. 2021 Nov;73:103634. doi: 10.1016/j.ebiom.2021.103634. Epub 2021 Oct 18. PubMed PMID: 34673450; PubMed Central PMCID: PMC8528684.

Humayun M, Ayuso JM, Brenneke RA, Virumbrales-Muñoz M, Lugo-Cintrón K, Kerr S, Ponik SM, Beebe DJ. Elucidating cancer-vascular paracrine signaling using a human organotypic breast cancer cell extravasation model. Biomaterials. 2021 Mar;270:120640. doi: 10.1016/j.biomaterials.2020.120640. Epub 2021 Feb 6. PubMed PMID: 33592387; PubMed Central PMCID: PMC9703973.

Ayuso JM, Park KY, Virumbrales-Muñoz M, Beebe DJ. Toward improved in vitro models of human cancer. APL Bioeng. 2021 Mar;5(1):010902. doi: 10.1063/5.0026857. eCollection 2021 Mar. PubMed PMID: 33532672; PubMed Central PMCID: PMC7822630.

Ayuso JM, Rehman S, Virumbrales-Munoz M, McMinn PH, Geiger P, Fitzgerald C, Heaster T, Skala MC, Beebe DJ. Microfluidic tumor-on-a-chip model to evaluate the role of tumor environmental stress on NK cell exhaustion. Sci Adv. 2021 Feb;7(8). doi: 10.1126/sciadv.abc2331. Print 2021 Feb. PubMed PMID: 33597234; PubMed Central PMCID: PMC7888951.

Virumbrales-Muñoz M, Paz-Artigas L, Ciriza J, Alcaine C, Espona-Noguera A, Doblaré M, Sáenz Del Burgo L, Ziani K, Pedraz JL, Fernández L, Ochoa I. Force Spectroscopy Imaging and Constriction Assays Reveal the Effects of Graphene Oxide on the Mechanical Properties of Alginate Microcapsules. ACS Biomater Sci Eng. 2021 Jan 11;7(1):242-253. doi: 10.1021/acsbiomaterials.0c01382. Epub 2020 Dec 18. PubMed PMID: 33337130.

Ayuso JM, Rehman S, Farooqui M, Virumbrales-Muñoz M, Setaluri V, Skala MC, Beebe DJ. Microfluidic Tumor-on-a-Chip Model to Study Tumor Metabolic Vulnerability. Int J Mol Sci. 2020 Nov 28;21(23). doi: 10.3390/ijms21239075. PubMed PMID: 33260673; PubMed Central PMCID: PMC7730115.

Virumbrales-Muñoz M, Chen J, Ayuso J, Lee M, Abel EJ, Beebe DJ. Organotypic primary blood vessel models of clear cell renal cell carcinoma for single-patient clinical trials. Lab Chip. 2020 Nov 24;20(23):4420-4432. doi: 10.1039/d0lc00252f. PubMed PMID: 33103699; PubMed Central PMCID: PMC8743028.

Virumbrales-Muñoz M, Ayuso JM, Gong MM, Humayun M, Livingston MK, Lugo-Cintrón KM, McMinn P, Álvarez-García YR, Beebe DJ. Microfluidic lumen-based systems for advancing tubular organ modeling. Chem Soc Rev. 2020 Sep 1;49(17):6402-6442. doi: 10.1039/d0cs00705f. Review. PubMed PMID: 32760967; PubMed Central PMCID: PMC7521761.

Lugo-Cintrón KM, Ayuso JM, White BR, Harari PM, Ponik SM, Beebe DJ, Gong MM, Virumbrales-Muñoz M. Matrix density drives 3D organotypic lymphatic vessel activation in a microfluidic model of the breast tumor microenvironment. Lab Chip. 2020 May 7;20(9):1586-1600. doi: 10.1039/d0lc00099j. Epub 2020 Apr 16. PubMed PMID: 32297896; PubMed Central PMCID: PMC7330815.

Lugo-Cintrón KM, Gong MM, Ayuso JM, Tomko LA, Beebe DJ, Virumbrales-Muñoz M, Ponik SM. Breast Fibroblasts and ECM Components Modulate Breast Cancer Cell Migration Through the Secretion of MMPs in a 3D Microfluidic Co-Culture Model. Cancers (Basel). 2020 May 6;12(5). doi: 10.3390/cancers12051173. PubMed PMID: 32384738; PubMed Central PMCID: PMC7281408.

Virumbrales-Muñoz M, Ayuso JM, Lacueva A, Randelovic T, Livingston MK, Beebe DJ, Oliván S, Pereboom D, Doblare M, Fernández L, Ochoa I. Author Correction: Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling. Sci Rep. 2020 Jan 15;10(1):757. doi: 10.1038/s41598-020-57830-0. PubMed PMID: 31937889; PubMed Central PMCID: PMC6960145.

Virumbrales-Muñoz M, Santos-Vizcaino E, Paz L, Gallardo-Moreno AM, Orive G, Hernandez RM, Doblaré M, Gonzalez-Martin ML, Fernández LJ, Pedraz JL, Ochoa I. Force spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in coated alginate microspheres. Sci Rep. 2019 Dec 27;9(1):20112. doi: 10.1038/s41598-019-56547-z. PubMed PMID: 31882828; PubMed Central PMCID: PMC6934587.

Virumbrales-Muñoz M, Livingston MK, Farooqui M, Skala MC, Beebe DJ, Ayuso JM. Development of a Microfluidic Array to Study Drug Response in Breast Cancer. Molecules. 2019 Nov 30;24(23). doi: 10.3390/molecules24234385. PubMed PMID: 31801265; PubMed Central PMCID: PMC6930663.

Ayuso JM, Virumbrales-Munoz M, McMinn PH, Rehman S, Gomez I, Karim MR, Trusttchel R, Wisinski KB, Beebe DJ, Skala MC. Tumor-on-a-chip: a microfluidic model to study cell response to environmental gradients. Lab Chip. 2019 Oct 9;19(20):3461-3471. doi: 10.1039/c9lc00270g. PubMed PMID: 31506657; PubMed Central PMCID: PMC6785375.

Virumbrales-Muñoz M, Ayuso JM, Lacueva A, Randelovic T, Livingston MK, Beebe DJ, Oliván S, Pereboom D, Doblare M, Fernández L, Ochoa I. Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling. Sci Rep. 2019 Apr 17;9(1):6199. doi: 10.1038/s41598-019-42529-8. PubMed PMID: 30996291; PubMed Central PMCID: PMC6470149.

Jiménez-Torres JA, Virumbrales-Muñoz M, Sung KE, Lee MH, Abel EJ, Beebe DJ. Patient-specific organotypic blood vessels as an in vitro model for anti-angiogenic drug response testing in renal cell carcinoma. EBioMedicine. 2019 Apr;42:408-419. doi: 10.1016/j.ebiom.2019.03.026. Epub 2019 Mar 20. PubMed PMID: 30902740; PubMed Central PMCID: PMC6491391.

Burić SS, Podolski-Renić A, Dinić J, Stanković T, Jovanović M, Hadžić S, Ayuso JM, Virumbrales-Muñoz M, Fernández LJ, Ochoa I, Pérez-García VM, Pešić M. Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo. Oxid Med Cell Longev. 2019;2019:3061607. doi: 10.1155/2019/3061607. eCollection 2019. PubMed PMID: 30984333; PubMed Central PMCID: PMC6432727.

Livingston MK, Morgan MM, Daly WT, Murphy WL, Johnson BP, Beebe DJ, Virumbrales-Muñoz M. Evaluation of PEG-based hydrogel influence on estrogen receptor driven responses in MCF7 breast cancer cells. ACS Biomater Sci Eng. 2019;5(11):6089-6098. doi: 10.1021/acsbiomaterials.9b00480. PubMed PMID: 31942444; PubMed Central PMCID: PMC6961958.

Ayuso JM, Truttschel R, Gong MM, Humayun M, Virumbrales-Munoz M, Vitek R, Felder M, Gillies SD, Sondel P, Wisinski KB, Patankar M, Beebe DJ, Skala MC. Evaluating natural killer cell cytotoxicity against solid tumors using a microfluidic model. Oncoimmunology. 2019;8(3):1553477. doi: 10.1080/2162402X.2018.1553477. eCollection 2019. PubMed PMID: 30723584; PubMed Central PMCID: PMC6350694.

Mármol I, Virumbrales-Muñoz M, Quero J, Sánchez-de-Diego C, Fernández L, Ochoa I, Cerrada E, Yoldi MJR. Alkynyl gold(I) complex triggers necroptosis via ROS generation in colorectal carcinoma cells. J Inorg Biochem. 2017 Nov;176:123-133. doi: 10.1016/j.jinorgbio.2017.08.020. Epub 2017 Aug 31. PubMed PMID: 28892675.

Virumbrales-Muñoz M, Ayuso JM, Olave M, Monge R, de Miguel D, Martínez-Lostao L, Le Gac S, Doblare M, Ochoa I, Fernandez LJ. Multiwell capillarity-based microfluidic device for the study of 3D tumour tissue-2D endothelium interactions and drug screening in co-culture models. Sci Rep. 2017 Sep 20;7(1):11998. doi: 10.1038/s41598-017-12049-4. PubMed PMID: 28931839; PubMed Central PMCID: PMC5607255.

Ayuso JM, Monge R, Martínez-González A, Virumbrales-Muñoz M, Llamazares GA, Berganzo J, Hernández-Laín A, Santolaria J, Doblaré M, Hubert C, Rich JN, Sánchez-Gómez P, Pérez-García VM, Ochoa I, Fernández LJ. Glioblastoma on a microfluidic chip: Generating pseudopalisades and enhancing aggressiveness through blood vessel obstruction events. Neuro Oncol. 2017 Apr 1;19(4):503-513. doi: 10.1093/neuonc/now230. PubMed PMID: 28062831; PubMed Central PMCID: PMC5464359.

Ayuso JM, Virumbrales-Muñoz M, Lacueva A, Lanuza PM, Checa-Chavarria E, Botella P, Fernández E, Doblare M, Allison SJ, Phillips RM, Pardo J, Fernandez LJ, Ochoa I. Development and characterization of a microfluidic model of the tumour microenvironment. Sci Rep. 2016 Oct 31;6:36086. doi: 10.1038/srep36086. PubMed PMID: 27796335; PubMed Central PMCID: PMC5086897.

Ciriza J, Saenz del Burgo L, Virumbrales-Muñoz M, Ochoa I, Fernandez LJ, Orive G, Hernandez RM, Pedraz JL. Graphene oxide increases the viability of C2C12 myoblasts microencapsulated in alginate. Int J Pharm. 2015 Sep 30;493(1-2):260-70. doi: 10.1016/j.ijpharm.2015.07.062. Epub 2015 Jul 26. PubMed PMID: 26220651.

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